O3Vets 20-250 LBS Animal Protocol
Items to Consider:
- Approximately 1 ml of blood/saline mixture should be used per pound.
- Mixture will be 80% – 90% saline, 10% – 20% blood
- Run mixture at 6 ml/minute past UV light using peristaltic IV pump with the Chimp UV or 12 ml/min using the Gorilla UV.
- The 6″ Chimp cuvette holds about 18 ml of fluid.
- 6” Chimp cuvette
- 0.9% Saline Solution
- butterfly needle
- hypodermic needle
- 30 – 60 cc syringe
- Clamp (plastic hemostat)
- Insulin syringe for anticoagulant
Step 1: DVD (3:30) Draw saline into syringe and infuse into blood bag. Note: You will need more than one syringe full if treating an animal over 55 lbs.
Step 2: DVD (4:30) Before drawing blood, load syringe with heparin using small insulin syringe.
Step 3: DVD (5:15) Withdraw blood from patient.
Step 4: DVD (5:25) Infuse mixture (or just blood if you have already infused the saline) into the blood bag.
Step 5: DVD (5:40) Clamp off on blood bag luer lock port (bottom right) and connect cuvette. Note: If gravity doesn’t fill line, connect a syringe to the other side of the line and draw fluid through.
Step 6: DVD (7:30) Remove clamp, remove cap on cuvette and hold cuvette upright as the line is primed.
Step 7: DVD (7:50) Insert line into infusion pump and cuvette into machine and turn Chimp UV on.
Step 8: DVD (8:45) Connect the other cuvette luer lock to the fill port (Bottom Left) of the O3Vets bag to form a closed loop system.
Step 9: DVD (8:56) Set the amount of fluid being pumped at 360 ml/h and start pump to begin circulating the mixture.
Step 10: DVD (10:00) When pumping time is complete turn off UV unit, and then infuse ozone into bag.
Step 11: DVD (11:20) Remove line from pump and Chimp UV and clamp off to Left port on blood bag.
Step 12: DVD (ll:45) Crimp cuvette line and connect syringe to luer lock. Draw up mixture into syringe to reinfuse into the patient.
Step 13: Properly dispose of blood products.