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June 20, 2022 9 min read

Have you ever attendedan all-day online summit packed full of got-to-have information that left you with questions?

Have you ever missed out on an all-day online summit packed full of got-to-have information that left you with questions? 

If either situation is you, then this blog is a got-to-read just for you. It contains a slew of questions and answers from a recent summit hosted by O3Vets on ozone therapy. Veterinarians from around the globe gathered online to educate viewers about ozone therapy and to answer some of the questions everyday people have about ozone therapy. 

It's important to note that the following information reflects the view of the various veterinarians who participated in our event and may not be the opinion of O3Vets.

Our panel of experts included:

  • Dr. Margo Roman
  • Dr. Marty Langhofer
  • Dr. Jyl Rubin
  • Dr. Marlene Siegel

Q: Can Ozone Injections Help Reduce Chronic Scar Tissue?

 A: Yes! Injections of Prolo+Ozone and a little bit of cortisol can help with scar tissue. The injection is given under the scar, and it literally lifts the scar up. Because ozone is supporting the fascia and oxygenating it, the scar heals up nicely.


Q: When Treating A Small Animal With A Combination Of Ozone And Acupuncture, How Much Ozone Should Be Used In Each Acupuncture Point? 

A: The amount of ozone to be used in an acupuncture point depends on which acupuncture point is being treated. If the acupuncture point is on a small animal’s back, 3ml to 5ml of ozone would be appropriate. If the acupuncture point is on a limb of a small animal, 1ml to 2ml of ozone can be used. If more ozone is going to be used, it would be a good idea to reduce the ozone concentration level from 35ug/ml to a lower concentration.


Q: Can Ozone Be Used To Treat Melanoma In Horses? If So, How Can It Be Used?

A: Ozone can be used to treat melanoma in horses!! In fact, ozone is definitely worth a try when it comes to melanoma because there are no other available treatments, conventional or otherwise. By using ozone, the cancerous tumors have a shot at being controlled for quite some time.

 Unfortunately, cancer involves enslaved monocytes, which actually work to protect diseased tumors. Ozone is one way of directing those monocytes to stop protecting cancerous tumors and do what they are supposed to do, which is fighting off germs and eliminating infectious cells.

 There is no need to inject the melanoma cells themselves with ozone because those cells have a tendency to rot and attract flies. However, ozone can be administered using DIV. Administering ozone therapy this way provides great blood supply and allows the white blood cells to leave the capillaries and go into the tissue, which creates abscesses to block off the ozone. The cancer cells will die as a result of this.

Q: Is Ozone Therapy Recommended In Foals With High Lactate Levels And Systemic Inflammatory Response Syndrome (SIRS)? If So, How Is It Managed?

A: Ozone therapy can be good for any animal with inflammatory or immune-suppressant disease.

Conventional treatments often rely on steroids, which are not the answer because they suppress the immune system in an already immunocompromised animal. Foals with SIRS seem to respond so much better to ozone than to conventional treatments.

When the body is in a sympathetic state, all of its repair mechanisms and detoxification mechanisms are shut down. In these cases, it is vital to get those animals in a parasympathetic state, which ozone facilitates.


Q: What Is The Best Way To Ozonate Saline To Be Administered Intravenously?

A: Ozonated saline isn’t commonly administered DIV unless it’s given to a large animal (horse, cow, etc.). It is much better to inject straight ozone into different parts of the body or inject sub-q fluids rather than administering ozone DIV if treating a smaller animal. However, if ozonated saline is administered this way, it is a good idea to use a water blanket underneath the patient with warm towels to keep the patient free of becoming cold.

Q: When Ozonating Saline, Can The Ozone Be Injected Directly Into The Bag Of Saline? 

A:It is not recommended that ozone be injected directly into a bag of saline. Rather, an ozone fluid bubbler should be used whenever ozonating saline or water. The potency of the ozone can break down the bag itself.

The rule of thumb for bubbling fluids is to bubble the fluid for 5 minutes for every 250ml of fluid in the bubbler.


Q: What Problems Have Or Could Be Associated With Ozone Therapy?

A: Just as with any medical treatment in veterinary or human medicine, occasionally a negative result can occur when using ozone, but O3Vets’ panel of experts had the following to say:

Treating an Animal’s Bladder with Ozone - Treating bladder infections and cancers of the bladder with ozone can occasionally present problems. An ultrasound of the bladder should always be done before administering ozone to the bladder. Additionally, the bladder should be drained completely before an ozone treatment, and a lower concertation of ozone should be used for this type of treatment.

Nebulizing Ozone with Oil - When nebulizing ozone through oil, it is imperative to make sure that the oil is bubbling well and is not thick and goopy. If the oil is not bubbling properly, it can cause issues. It is also vital that ozone be bubbled through OIL and not WATER when being nebulized. The only way ozone is safe to breathe in is by being bubbled through oil. This turns the oil into ozonides and peroxides, which are safe to breathe.

Adverse Responses to Ozone - If ozone is given and an animal reacts poorly or has a bad adverse ataxic response, giving Vitamin C orally or homeopathic Carcinosin with coconut water or maple syrup may help.

Ozone is a Gas – Because ozone is a gas, special care should always be taken when administering ozone therapy. If, at any time, ozone is smelled during an ozone treatment, the equipment should be turned off and checked to make sure it is hooked up correctly and that the proper protocol is being followed.


Q: When Limb Bagging, Should The Entire Bag Be Filled Up At Once?

A: First, it is important that a destruct always be used when limb bagging!

 It is not necessary to completely fill a limb bag when administering ozone this way. A bag should not be filled to the point that it bulges excessively or looks like it’s going to break or pop.



Q: How Can Ozone Be Used On Cats With FIP?

A: FIP in cats is a very challenging disease, so multiple modalities are usually necessary to treat it.

Before treating a cat with FIP, it is suggested that blood be drawn to check for co-diseases like bartonella. Many times, these cases have multiple diseases. 

Any cat with FIP should be on a proper diet, and its immune system should be built up as much as possible.

Some of the ways ozone can be used in a FIP cat patient include rectal ozone, ozone flushes, MAHT, and nebulizing ozone.

Q: How Well Do Cats Tolerate Rectal Ozone?

A: Cats, like all animals, vary from cat to cat. Some tolerate rectal ozone just fine, and others don’t. 

If a cat is being treated with ozone and seems to be struggling with holding still, etc., a little bit of Isoflurane can help. Another option for a stressed out kitty is putting him/her into a loop lounge for about 15 minutes before the treatment.

Q: Once A Cat Is Brought To A Clinic With Lower Urinary Tract Disease, How Can Ozone Help?

A: Treating lower urinary tract disease in cats always depends on the underlying factors. For example, is the animal eating a species inappropriate diet? Does the cat have Vitamin D deficiency? Is there inflammation in other areas of the body? What is the home environment like? Is the cat sharing a litterbox? How much water is he/she drinking per day? How old is the cat? And the list goes on.

As mentioned above, a very thorough workup should be done whenever a cat presents with lower urinary tract disease. This should encompass a lot of bloodwork, a urine check/culture, and an ultrasound. Once all diagnostics have been completed, the treatment regimen can begin, starting with getting the inflammation under control.



Q:How Can Young Dogs With Urinary Incontinence Be Treated With Ozone?

A: As in most cases, when a dog is brought to a vet clinic with urinary incontinence, a thorough workup should be done to determine the cause of the incontinence. The workup should include x-rays to check for bladder stones. Once the workup is completed, a treatment plan can be implemented.

 There are some Chinese herbs that can be helpful for incontinence, and these can be administered along with ozone therapy.



Q: Is It Recommended To Use Antioxidants When Doing Ozone Therapy? If So, What Dose of Ozone Is Used?

A: If antioxidants are going to be given along with ozone therapy, it is recommended that the ozone treatment be given first followed by the antioxidants. The reason for this is because a large flush of antioxidants all at once can scavenge the ozone, which leads to a potential ineffective ozone treatment due to there not being enough ozone left in the system.

Unfortunately, there are no studies that indicate how soon after an ozone treatment that antioxidants should be given. The responses to this question range from 30 minutes to 1 day.



 Q: What If Ozone Therapy Doesn’t Provide The Results You Anticipated?

A: The following steps should be taken for any and all treatments at a veterinarian clinic:

  • ALL pet parents should sign consent forms before beginning any type of diagnostic testing or treatment. This should be a mandatory step one before anything else is done!
  • As mentioned above, a thorough workup should be conducted on any animal. This can involve any or all (and more) of the following: bloodwork, x-rays, ultrasound, CT scan, complete history, etc.
  • Before communicating any type of treatment plan to a pet parent, a vet should make sure there is a concrete diagnosis.
  • The treatment plan itself should be communicated clearly and concisely to pet parents, and they should be able to ask any and all questions they may have.
  • When a treatment plan is communicated to a pet parent, a veterinarian should NEVER over promise any type of result from ozone therapy or any other treatment.
  • If ozone therapy or other treatments are not going as anticipated, pet parents should be told that further diagnostics will need to be done, and the course of action may need to be changed.
  • If a veterinarian makes a mistake when treating an animal, he/she should admit the mistake and take ownership of it. If, God forbid, a patient should pass during a treatment, a veterinarian should insist that the animal not be permitted to leave the clinic without first performing a necropsy of the animal. This could make or break a lawsuit should one result from a veterinarian’s mistake.
  • It is also important to document everything! Taking pictures and videos before, during, an after treatments goes a long way.
  • Making sure you have a phone service that records telephone calls is also a good idea in case there is miscommunication at some point.



Q: What Marketing Ideas Can Be Used To Promote Ozone Therapy In A Rural Area Where Alternative Medicine Is Not Popular?

 A: As a holistic or integrative veterinarian, it is very important to get the word out about ozone therapy!

 First, it is vital that a vet starting with ozone therapy be open to listening to the community and be able to ask community members about their wants and needs for their animals.

 Second, ozone therapy does not have to be labeled “alternative” medicine. That word sometimes scares people who only know about traditional, conventional medicine.

There are numerous ways that ozone therapy can be promoted and marketed in rural (or any geographical) areas including the following:

  • Volunteering to talk on local radio or TV shows
  • Attending community events and handing out flyers, brochures, etc. at each event
  • Attending SPCA events and animal fundraisers
  • Donating a small gift of ozone products for raffles and other money-raising opportunities
  • Hosting an educational event in your facility for customers who are already established clients
  • Hiring a marketing expert or taking a marketing course to help promote ozone therapy
  • Ensuring that your website is clear, concise, and informative for people who know nothing about ozone therapy.
  • Attending dog shows
  • Asking new clients who experience ozone therapy to spread the word. Word of mouth is vital, especially is smaller, rural communities where everybody knows everybody



 O3Vets hosts an Ozone Therapy Summit each year. Each of its summits contains a Q&A segment. Rather than wait for a blog to be published with that Q&A, why not tune in live or online for the next Summit? Keep your eyes peeled on your email and on O3Vets’ website for an announcement regarding the next Ozone Therapy Summit so you can ask your own questions about ozone therapy and its never-ending uses. Til then!

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